Ratchet and Clank had some good humor, even though sometimes it may have seemed like it was trying a little too hard, I definitely enjoyed it for the most part.

No More Heroes is pretty humorous too. Just charging up your beam sword is hilarious on its own...

And I gotta say Metal Gear Solid and its sequels/prequel, its type of humor is great. "You've created a time paradox!"

Thanks to GLaDOS, Portal can be pretty hilarious, usually in a morbid sense. Especially during the final battle. Tellin' it like it is. No mercy man.